Part:BBa_K1103004
NFκB-IκB-GFP
NF-κB Binding Element can recognize and bind with the free NF-κB. NF-κB regulates the expression of downstream genes by binding with the element. IκB is the inhibitor of NF-κB. The activity of NF-κB will be large-sized decreased, when IκB binds with NF-κB. The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. We fuse the fragments of IκB and GFP. The two proteins can co-express. We combined these three sequences(NF-κB, IκB, GFP) into a specific sequence by using overlap PCR method, thus, the recombinant fragment could provides its significant bio-effects.
IκB fragment has two PstI restriction sites. We tried to use point mutation method to mutant a A into T of PstI, but it hardly work because of its functionality lost.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 225
Illegal PstI site found at 408 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 225
Illegal PstI site found at 408 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 75
Illegal XhoI site found at 56
Illegal XhoI site found at 62
Illegal XhoI site found at 240 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 225
Illegal PstI site found at 408 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 225
Illegal PstI site found at 408 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 376
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